mouse anti tubβ3  (Santa Cruz Biotechnology)

Journal: Frontiers in Immunology

Article Title: A cell line–derived, immune-competent neurospheroid model to study neuroinflammation and human brain disorders

doi: 10.3389/fimmu.2026.1792896

Figure Lengend Snippet: SH-SY5Y and U138 MG cell differentiation into neuron-like (NLCs) and astrocyte-like cells (ALCs). (A, B) Representative immunofluorescence images of β3-tubulin (in yellow) and Nestin (in green) for undifferentiated SH-SY5Y cells (Ctrl) and NLCs differentiated on plastic (Diff.) or on Geltrex (Diff. on Geltrex). Nuclei were counterstained with DAPI (blue). (C, D) Quantification of the fluorescence intensity for β3-tubulin and Nestin normalized to DAPI signals. (E) Relative mRNA expression levels of the proneural transcription factors Mash1 , Nurr1 , and NeuroD1 in undifferentiated and differentiated SH-SY5Y cells. (F) Representative S100B (in red) immunofluorescence images for undifferentiated U138 MG cells or ALCs differentiated on plastic (Diff.) or on Geltrex (Diff. on Geltrex). (G, H) Quantification of the U138 MG proliferating fractions in differentiated and control conditions measuring the EdU-incorporating cells. (I) Quantification of the S100B fluorescence intensity normalized to DAPI signals. Data are shown as mean ± SD from a minimum of three independent replicates (n ≥ 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test. *p< 0.03; **p< 0.02; ***p< 0.0002; ****p< 0.0001. Scale bar: 100μm. FI, Fluorescence intensity; Mash1, Mammalian achaete-scute homolog 1; NeuroD1, Neuronal differentiation 1; Nurr1, Nuclear receptor related 1 protein.

Article Snippet: β3-Tubulin , 2G10 , Mouse , sc-80005 , Santa Cruz Biotechnology, Inc., Dallas, TX, USA , 2 μg/mL.

Techniques: Cell Differentiation, Immunofluorescence, Fluorescence, Expressing, Control

Journal: Frontiers in Immunology

Article Title: A cell line–derived, immune-competent neurospheroid model to study neuroinflammation and human brain disorders

doi: 10.3389/fimmu.2026.1792896

Figure Lengend Snippet: Formation and morphological assessment of bi-hNSPHs. (A) Representative bright-field microscopy images showing the formation and growth of bi-hNSPHs within 14 days. Scale bar: 200 µm. (B) Growth curves based on the measurement of bi-hNSPHs areas (µm²) and diameters (µm) every other day. (C) H&E staining of bi-hNSPHs cryosection at 14 days to reveal their internal cellular disposition. Scale bar: 100 µm. (C) Representative immunofluorescence images of neuronal markers for SH-SY5Y – β3-tubulin in yellow, Synapsin in red, MAP2 in magenta and Nestin in green – and astrocytic markers for U138 MG – S100B in red. (D) MAP2, Microtubule associated protein 2.

Article Snippet: β3-Tubulin , 2G10 , Mouse , sc-80005 , Santa Cruz Biotechnology, Inc., Dallas, TX, USA , 2 μg/mL.

Techniques: Microscopy, Staining, Immunofluorescence

Journal: Frontiers in Immunology

Article Title: A cell line–derived, immune-competent neurospheroid model to study neuroinflammation and human brain disorders

doi: 10.3389/fimmu.2026.1792896

Figure Lengend Snippet: Internal composition of tri-hNSPHs. (A) Representative immunofluorescence images of tri-hNSPH cryosections at 4, 7, 9, 11, and 14 days of culture. Sections were stained for the astrocyte marker S100B (in red), the neuronal marker β3-tubulin (in yellow), and the microglia/macrophage marker Iba-1 (in cyan). Nuclei were counterstained with DAPI (blue). (B) Representative immuno-fluorescence images of neuronal – Synapsin in red, MAP2 in magenta and Nestin in green – and microglial – HLA-DR in red – markers on cryosectioned tri-hNSPHs. Scale bar: 50-100 µm. Iba-1, Ionized calcium-binding adapter molecule 1; MAP2, Microtubule associated protein 2.

Article Snippet: β3-Tubulin , 2G10 , Mouse , sc-80005 , Santa Cruz Biotechnology, Inc., Dallas, TX, USA , 2 μg/mL.

Techniques: Immunofluorescence, Staining, Marker, Fluorescence, Binding Assay